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Restriction site mutation analysis, a proposed methodology for the detection and study of DNA base changes following
J M Parry1, M Shamsher, D O Skibinski
1Molecular Biology Research Group, School of Biological Sciences, University College of Swansea, Singleton Park, UK.
Abstract:
We propose a methodology for the detection of DNA base changes in restriction enzyme recognition sites. The restriction site mutation (RSM) technique is based upon the detection of DNA sequences resistant to the cutting action of specific restriction enzymes and the amplification of these resistant sequences using the polymerase chain reaction. As outlined, the RSM method has the potential for use in the study of induced base changes in any species, tissue and genes of known DNA sequence for which unique DNA primers are available, and which contains a number of unique restriction enzyme recognition sites.