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Updated: May 28, 2026
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
Repair of large segmental bone defects using bone marrow stromal cells with demineralized bone matrix
Jian-zhong Xu1, Hui Qin, Xu-quan Wang
1Department of Orthopaedics, Southwest Hospital, Tissue Engineering Center of the Third Military Medical University, Chongqing, China.
Objective:
The aim of the present study was to evaluate the effect of tissue-engineered constructs on repair of large segmental bone defects in goats.
Methods:
Allogenic demineralized bone matrix (aDBM) was seeded with autologous marrow stromal cells (aMSC) for seven days to construct DBM-MSC grafts prior to implantation. 24 goats were randomly divided into three groups (eight in each). In each group, 3 cm diaphyseal femoral defects were created unilaterally, and subsequently filled with the DBM-MSC grafts, DBM alone and an untreated control, respectively. Radiological analysis and biomechanical evaluation were performed at 12 and 24 weeks after operation.
Results:
Obvious increases in radiological scoring and biomechanical strength were found in the DBM-MSC group when compared to the DBM group. X-ray examination showed excellent bone healing in the DBM-MSC group, whereas only partial bone repair was seen in the DBM group, and no healing in untreated controls. Histologically, a tendency to bone regeneration and remodeling was far more obvious for the DBM-MSC group than the DBM only and untreated controls.
Conclusion:
Our results strongly suggest that transplantation of bone MSC within a DBM could have advantages for the bone repair of large segmental defects.
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