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Updated: May 28, 2026
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In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
Clathrin is required for Scar/Wave-mediated lamellipodium formation
Jérémie J Gautier1, Maria E Lomakina, Lamia Bouslama-Oueghlani
1CNRS UPR3082, Laboratoire d'Enzymologie et Biochimie Structurales, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
Journal of Cell Science
|October 20, 2011
Summary
Clathrin heavy chain (CHC) regulates cell migration by controlling actin network formation. This study reveals CHC
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- The Scar/Wave complex (SWC) is crucial for generating lamellipodia via Arp2/3-dependent actin polymerization.
- Identifying novel regulators of SWC activity is essential for understanding cell migration dynamics.
Purpose of the Study:
- To identify new regulators of the Scar/Wave complex (SWC).
- To elucidate the role of Clathrin heavy chain (CHC) in lamellipodium formation and cell migration.
Main Methods:
- Proteomics and functional genomics screen in Drosophila cells.
- Experimental manipulation of Clathrin heavy chain (CHC) localization and expression.
- Analysis of SWC membrane recruitment, protrusion velocity, and cell migration.
Main Results:
- Clathrin heavy chain (CHC) was identified as a protein interacting with the SWC.
- CHC depletion impaired lamellipodium formation, independent of its role in membrane trafficking.
- Altering CHC localization modulated SWC membrane recruitment, affecting protrusion velocity and cell migration.
Conclusions:
- Clathrin heavy chain (CHC) plays a novel role in controlling lamellipodium formation.
- CHC facilitates the recruitment of the SWC to the plasma membrane, thereby regulating cell migration.
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