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Updated: May 28, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Lipid peroxidation in the first trimester of pregnancy
Branislava Jakovljevic1, Aleksandra Novakov-Mikic, Snezana Brkic
1Department of obstetrics and gynecology, Clinical center Vojvodina, Branimira Cosica, Novi Sad, Serbia.
Aim:
To determine the existence and level of oxidative stress caused by lipid peroxidation in pregnancy.
Methods:
The research was conducted as prospective examination that included 60 healthy women (age 18-45). The women included in the examination were divided into two groups. The group I (N-31) included women in the first trimester of normal, healthy pregnancy. The group II included healthy nongravid women (N-29). Concentrations of markers of lipid peroxidation malondialdehyde (MDA) and thiobarbituric acid (TBARS) were determined using commercial ELISA tests OxiSelect(™) TBARS Assay Kit and OxiSelect(™) MDA ELISA Kit.
Results:
The results of this research indicate that the concentrations of the markers of lipid peroxidation TBARS and MDA are detectable in both groups. Higher mean values of MDA (>20 pmol/mg) were measured in the group of pregnant women, than in the group of nongravid women. The results indicate that mean values of TBARS markers are lower in pregnant women (≤50 µM) than in nongravid women (>100 µM).
Conclusion:
The marker of lipid peroxidation MDA proved to be a sensitive marker for following lipid peroxidation during pregnancy, therefore it can be considered as a good predictor of possible complications during pregnancy.
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