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Perforated dried blood spots: a novel format for accurate microsampling.

Fumin Li1, John Zulkoski, Douglas Fast

  • 1Covance Laboratories, Inc., Bioanalytical Chemistry, 3301 Kinsman Boulevard, Madison, WI 53704, USA. fumin.li@covance.com

Bioanalysis
|October 21, 2011
PubMed
Summary

Perforated dried blood spots (PDBS) offer a novel microsampling method, overcoming limitations of traditional dried blood spots (DBS). This technique enhances bioanalysis accuracy and efficiency by enabling complete sample utilization and eliminating hematocrit effects.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Sciences
  • Pharmacokinetics

Background:

  • Traditional dried blood spots (DBS) present bioanalytical challenges, including sample waste, carryover risk from punching, and hematocrit (HCT) variability.
  • Current DBS methods often use only a fraction of the sample, impacting recovery assessments and accuracy.

Purpose of the Study:

  • To introduce and validate a novel microsampling technique using perforated dried blood spots (PDBS).
  • To address the limitations of conventional DBS, such as sample wastage, punching procedures, and HCT interference.

Main Methods:

  • Development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for lansoprazole quantification in human whole blood using PDBS.
  • Preparation of PDBS discs (6.35 mm diameter, 0.83 mm thickness) with precise blood sample volumes (5-10 µl).

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  • Analysis involved directly pushing PDBS samples into 96-well plates using single-use pipette tips, under Good Laboratory Practice (GLP) criteria.

    • Main Results:

    • PDBS demonstrated a viable alternative to conventional DBS, offering complete sample utilization.
    • The method eliminated the need for sample punching, reducing carryover concerns.
    • Recovery assessments were simplified, and the influence of HCT on quantitation accuracy was mitigated.

    Conclusions:

    • Perforated dried blood spots (PDBS) provide an improved microsampling approach for bioanalysis.
    • PDBS enhances accuracy, efficiency, and reliability in drug quantification, particularly by addressing HCT effects.
    • This novel technique offers significant advantages over traditional DBS for various bioanalytical applications.