Related Experiment Video
Updated: May 28, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Impact of internal standard addition on dried blood spot analysis in bioanalytical method development
Roland Meesters1, Gero Hooff, Nick van Huizen
1Laboratory of Neuro-Oncology & Clinical & Cancer Proteomics, Department of Neurology, University Medical Center Rotterdam (Erasmus MC), Rotterdam, The Netherlands. r.meesters@erasmusmc.nl
Background:
Addition of internal standards to dried blood spot (DBS) specimens can be complicated. Therefore, we studied the feasibility of different internal standard addition procedures. Nevirapine and its stable-isotope analogue were used as model compounds and concentrations in DBS specimen were determined by matrix-assisted laser desorption/ionization-triple quadrupole tandem mass spectrometry using selected reaction monitoring.
Results:
The addition procedure of the stable isotope-labeled internal standard had significant impact on observed nevirapine concentrations. Relative recovery rates depending on the internal standard addition procedure ranged between 11.4 and 107.9%. Experiments with different punch sizes (5 and 7 mm diameter) showed no significant influence on observed nevirapine concentrations.
Conclusion:
Application of internal standard prior to blood spotting provided good nevirapine recoveries and this procedure is well suited for applying DBS in infectious diseases, especially in HIV-infection treatment.
More Related Videos
Related Concept Videos
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Absorption Spectroscopy: Interference
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Data Validation
Key parameters for method validation include:

