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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot
Hanene Bouraoui1, Melek Ben Aissa, Feten Abbassi
1Unité de Biochimie et Biologie Moléculaire, Université de Tunis El Manar, Campus Universitaire, Tunis, Tunisia. hanenebouraoui@yahoo.fr
Abstract:
An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23(T), was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23(T) is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas chromatography were iso-C(15:0), iso-C(17:0) and iso C(17:1) ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA-DNA hybridization measurements revealed low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23(T) represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23(T) (=DSM 18496(T); LMG 23756(T)).
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