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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
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Published on: December 21, 2010

Photoactivated uranyl ion produces single strand breaks in plasmid DNA

Shannon A George1, Aaron M Whittaker, Diane M Stearns

  • 1Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, Arizona 86011, USA.

Chemical Research in Toxicology
|October 22, 2011
PubMed

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Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
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