Related Experiment Video
Updated: May 28, 2026

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
Published on: July 31, 2021
Unprecedented ultra-high-resolution hydroxy group (1)H NMR spectroscopic analysis of plant extracts
Pantelis Charisiadis1, Alexandra Primikyri, Vassiliki Exarchou
1Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, GR-45110, Greece.
Abstract:
A general method is demonstrated for obtaining ultra-high resolution in the phenolic hydroxy group 1H NMR spectroscopic region, in DMSO-d6 solution, with the addition of picric acid. Line-width reduction by a factor of over 100 was observed, which resulted in line-widths ranging from 1.6 to 0.6 Hz. This unprecedented resolution, in combination with the shielding sensitivity of the hydroxy group absorptions to substituent effects at least up to 11 bonds distant and the application of 2D 1H-13C HMBC techniques, allows the unequivocal structure analysis of natural products with phenolic hydroxy groups in complex plant extracts.
More Related Videos
11:49Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
Published on: March 24, 2016
09:37Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
Related Concept Videos
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
High-Resolution Mass Spectrometry (HRMS)
NMR Spectroscopy of Aromatic Compounds
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei in a...