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Updated: May 28, 2026

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Stabilization of DNA duplex by 2-substituted adenine as a minor groove modifier
Makiko Tanaka1, Ryota Kozakai, Yoshio Saito
1Department of Chemical Biology and Applied Chemistry, School of Engineering, Nihon University, Koriyama, Fukushima, Japan. makikot@ao.ce.nihon-u.ac.jp
Abstract:
2-(1-Naphthalenylethynyl)-2'-deoxyadenosine ((N)A) was synthesized and incorporated into oligodeoxynucleotides. DNA duplexes containing newly designed 5'-(N)AT-3'/3'-T(N)A-5' base pairs are considerably stabilized than unmodified duplexes by stacking interaction of naphthalene rings in the narrow minor groove as characterized by a new emission at longer wavelength and exciton coupled CD signals.
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