Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rheumatoid factors and glomerulonephritis.

M Miyazaki1, M Endoh, T Suga

  • 1Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.

Clinical and Experimental Immunology
|August 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of caspase cascade by HTLV-I tax through induction of NF-kappaB nuclear translocation.

Blood·1999
Same author

Regulation of synovial cell apoptosis by proteasome inhibitor.

Arthritis and rheumatism·1999
Same author

Long-term efficacy of immunization against hepatitis B virus in infants at high-risk analyzed by polymerase chain reaction.

Vaccine·1999
Same author

Psychological impact of informed consent in hospitalized cancer patients. A sequential study of anxiety and depression using the hospital anxiety and depression scale.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·1999
Same author

FK506 markedly enhances apoptosis of antigen-stimulated peripheral T cells by down-regulation of Bcl-xL.

Transplantation·1999
Same author

Activation of caspase-8 in transforming growth factor-beta-induced apoptosis of human hepatoma cells.

Hepatology (Baltimore, Md.)·1999

Rheumatoid factors (RFs) are elevated in diffuse proliferative lupus nephritis (DPLN) and bind to glomeruli, suggesting a role in DPLN pathogenesis. RFs are not significantly implicated in other glomerulonephritis types studied.

Area of Science:

  • Nephrology
  • Immunology
  • Rheumatology

Background:

  • The pathogenic role of rheumatoid factors (RFs) in glomerulonephritis with immune deposits remains unclear.
  • RFs are autoantibodies targeting IgG, and their involvement in various kidney diseases requires investigation.

Purpose of the Study:

  • To investigate the presence and pathogenic role of RFs in different types of glomerulonephritis.
  • To compare RF levels and glomerular binding in diffuse proliferative lupus nephritis (DPLN), membranous lupus nephritis (MLN), IgA nephropathy (IgAN), and idiopathic membranous nephropathy (MN).

Main Methods:

  • Quantification of IgG, IgA, and IgM RF isotypes in patient sera using solid-phase fluorescence immunoassay.
  • Assessment of RF activity in glomerular immune deposits by examining the binding of FITC-conjugated human IgG and its Fc portion to renal biopsy specimens.

Related Experiment Videos

Main Results:

  • Significantly elevated levels of IgG, IgA, and IgM RFs were observed in the sera of patients with DPLN compared to MLN, IgAN, and MN.
  • Human IgG demonstrated binding to glomerular-bound immunoglobulin exclusively in DPLN, mediated by the Fc portion of IgG.
  • This binding was not observed in MLN, IgAN, or MN, indicating disease-specific interactions.

Conclusions:

  • RFs and IgG may play a significant role in the formation of immune deposits within glomeruli in DPLN.
  • These findings suggest RFs are involved in the pathogenesis of DPLN, but likely not in MLN, IgAN, or MN.