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Cissus quadrangularis augments IGF system components in human osteoblast like SaOS-2 cells
Sridhar Muthusami1, Ilangovan Ramachandran, Senthilkumar Krishnamoorthy
1Department of Endocrinology, Dr.ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, India.
Summary
Cissus quadrangularis extract positively influences the Insulin-like Growth Factor (IGF) system in human osteoblast cells. This study shows increased IGF-I, IGF-II, and IGF-IR expression, suggesting potential for bone health.
Area of Science:
- Bone biology and regenerative medicine
- Endocrinology and metabolic bone diseases
- Pharmacology and natural product research
Background:
- Osteoporosis is a major public health concern linked to high morbidity and mortality.
- Insulin-like Growth Factor (IGF) signaling is critical for bone development and remodeling.
- The effects of Cissus quadrangularis on the IGF system in bone cells are not well understood.
Purpose of the Study:
- To investigate the impact of ethanolic Cissus quadrangularis extract on IGF system components in human osteoblast-like SaOS-2 cells.
- To determine if C. quadrangularis modulates the expression of IGF-I, IGF-II, IGF-IR, and IGFBP-3.
Main Methods:
- Human osteoblast-like SaOS-2 cells were treated with ethanolic C. quadrangularis extract.
- Gene expression was analyzed using Reverse Transcription Polymerase Chain Reaction (RT-PCR).
- Protein and secreted factor levels were assessed via Immunoradiometric assays and Western blotting.
Main Results:
- C. quadrangularis extract significantly increased mRNA expression of IGF-I, IGF-II, and IGF-IR.
- Secreted levels of IGF-I, IGF-II, and IGFBP-3 were elevated in the conditioned medium.
- Protein levels of IGF-IR were notably increased in treated cells.
Conclusions:
- Ethanolic extract of C. quadrangularis demonstrates a positive regulatory effect on key components of the IGF system in human osteoblasts.
- These findings support the traditional use of C. quadrangularis for bone disorders and suggest a mechanism involving IGF signaling.
- Further research may explore C. quadrangularis as a therapeutic agent for osteoporosis and bone regeneration.
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