Related Experiment Video
Updated: May 28, 2026

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils
Published on: November 16, 2015
B type CpG-DNA suppresses poly(I:C)-induced BLyS expression and production in human tonsillar fibroblasts
Takechiyo Yamada1, Xuefan Jiang, Seita Kubo
1Department of Otorhinolaryngology, University of Fukui, Fukui, 910-1193, Japan. ymdtkcy@u-fukui.ac.jp
Abstract:
Although B lymphocyte stimulator (BLyS) has potent costimulatory effects on B cells, the details of BLyS-expression in tonsillar fibroblasts remain unexplored. We examined the effect of the Toll-like receptor (TLR) ligands on BLyS-expression in human tonsillar fibroblasts as well as the crosstalk that occurs among different TLR ligands. The expression of BLyS mRNA by tonsillar fibroblasts was strongly induced in the presence of polyinosinic-polycytidylic acid (poly(I:C)) that is a ligand, of TLR3. We also revealed that DNA containing CpG motifs (CpG-DNA), coding for a TLR9 ligand, markedly suppressed the poly(I:C)-induced mRNA expression and protein production of BLyS. B type CpG-DNA decreased the poly(I:C)-induced phosphorylation of inhibitor kappa B alpha (IκBα) and its degradation. Pre-incubation with nuclear factor kappa B (NF-κB) signaling inhibitors reduced the poly(I:C)-induced BLyS-expression. These results indicate that human tonsillar fibroblasts strongly induce BLyS-expression and production that can be inhibited by CpG-DNA and regulated through NF-κB signaling.
Related Concept Videos
Cell Specific Gene Expression
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Abnormal Proliferation
Inhibitors of Bacterial DNA Synthesis
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Induced Pluripotent Stem Cells
Somatic cells are...
