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Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin
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Systematic error in seed plant phylogenomics.

Bojian Zhong1, Oliver Deusch, Vadim V Goremykin

  • 1Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. bjzhong@gmail.com

Genome Biology and Evolution
|October 22, 2011
PubMed
Summary
This summary is machine-generated.

Phylogenetic analysis of seed plants reveals that Gnetales are closely related to Pinaceae, challenging previous assumptions. This finding, based on chloroplast DNA, refines our understanding of conifer evolution.

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Area of Science:

  • Evolutionary Biology
  • Phylogenetics
  • Molecular Systematics

Background:

  • The evolutionary relationships of Gnetales, a group of gymnosperms, remain poorly understood due to significant divergence from related taxa.
  • Previous phylogenetic studies have faced challenges in resolving the precise placement of Gnetales within seed plant evolution.

Purpose of the Study:

  • To investigate the evolutionary properties of conifer chloroplast DNA sequences.
  • To improve taxon sampling of Cupressophyta (non-Pinaceae conifers) for more robust phylogenetic analysis.
  • To resolve the enigmatic phylogenetic position of Gnetales within seed plants.

Main Methods:

  • Sequencing of three new chloroplast (cp) genomes from Southern Hemisphere conifers to enhance Cupressophyta sampling.
  • Application of a site pattern sorting criterion to assess compositional heterogeneity and heterotachy in cp genome sequences.
  • Evaluation of the fit of conifer chloroplast genome sequences to a general time reversible + G substitution model.

Main Results:

  • Non-time reversible properties in aligned chloroplast genome sequences of Gnetales were found to mislead phylogenetic reconstruction.
  • Exclusion of the 2,250 most variable sites from the concatenated alignment led to phylogenetic analyses supporting a close relationship between Gnetales and Pinaceae (the Gnepine hypothesis).
  • The study demonstrates the critical importance of the goodness of fit between substitution models and sequence data in phylogenomic analyses.

Conclusions:

  • The Gnepine hypothesis, suggesting a close evolutionary link between Gnetales and Pinaceae, is supported by analyses that account for sequence heterogeneity.
  • The developed analytical protocol offers a robust method for assessing the reliability of phylogenomic inferences.
  • Accurate phylogenetic reconstruction of seed plants necessitates careful consideration of substitution model adequacy and data characteristics.