Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 28, 2026

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

Published on: May 21, 2020

Pumilio-2 function in the mouse nervous system.

Henrike Siemen¹, Damien Colas, H Craig Heller

¹Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, California, United States of America.

|October 22, 2011

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Induced neural stem cells in neuroregeneration: Progress and clinical prospects.

Neural regeneration research·2026

Same author

Beyond Water, Rest, and Shade: Advancing Farmworker Heat Protection Through Partnership.

American journal of industrial medicine·2026

Same author

Recapitulation of plaque formation, tau pathology, and neurodegeneration in a human 3D matrix model of Alzheimer's disease.

Cell reports methods·2026

Same author

Nurturing eggs with hiPSC-derived cells to improve outcomes in in vitro fertilization.

Cell stem cell·2026

Same author

Effects of lipopolysaccharide on energy metabolism and immune cell activation in different microglia model systems.

Brain, behavior, and immunity·2026

Same author

Temporal Transcriptional Regulation of Human Neuronal Differentiation via Forward Programming.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026

Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026

Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026

Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026

Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026

Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026

See all related articles

Pumilio-2 (Pum2) deficiency in mice impairs memory, increases activity, and causes abnormal brain activity and seizures. This highlights Pum2

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Coordinated mRNA translation at the synapse regulates neuronal function.
Pumilio-2 (Pum2) is implicated in mammalian nervous system development and neuronal morphology in vitro.
The in vivo role of Pum2 in adult mammals remains largely unknown.

Purpose of the Study:

To investigate the in vivo functional and molecular consequences of Pum2 disruption in adult mice.
To assess the impact of Pum2 deficiency on neurophysiology, behavior, and gene expression.
To elucidate the role of Pum2 in neuronal excitability and central nervous system function.

Main Methods:

Utilized Pum2-deficient mice for in vivo studies.
Monitored brain activity using electroencephalography (EEG).

More Related Videos

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

Published on: August 3, 2012

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Published on: March 8, 2024

Related Experiment Videos

Last Updated: May 28, 2026

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior

Published on: May 21, 2020

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

Published on: August 3, 2012

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Time-Lapse Imaging of Migrating Neurons and Glial Progenitors in Embryonic Mouse Brain Slices

Published on: March 8, 2024

Assessed behavioral traits including memory, locomotor activity, and nesting.

Examined seizure susceptibility using pentylenetetrazole.

Performed quantitative gene expression profiling in the hippocampus.

Main Results:

Pum2-deficient mice exhibited deficits in spatial and object memory tasks.
Increased locomotor activity, decreased body weight, and impaired nesting behavior were observed.
Spontaneous EEG abnormalities and reduced seizure thresholds were evident.
Differential gene expression, including neuronal ion channels, was identified in the hippocampus.

Conclusions:

Pum2 plays a critical role in adult mammalian central nervous system function.
Pum2 is essential for maintaining normal neuronal excitability and behavioral responses.
Pum2 deficiency leads to significant neurophysiological and behavioral alterations, impacting memory and environmental adaptation.