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Updated: May 28, 2026
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
[Effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto]
Lijing Chen1, Guoping Liao, Yan Wang
1Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. lijing-c@hotmail.com
Objective:
To investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.
Method:
The mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.
Result:
The QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).
Conclusion:
QKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.
