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[Quantitative determination of contents of three components in Brucea javanica by HPLC]
Zhongliu Zhou1, Renbing Shi, Bin Liu
1Chemistry Science and Technology School, Zhanjiang Normal University, Zhanjiang 524048, China. zhou110zhong99@sohu.com
Objective:
To develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.
Method:
The determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.
Result:
Linearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.
Conclusion:
The determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.
