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Improving peak capacity in fast online comprehensive two-dimensional liquid chromatography with post-first-dimension
Marcelo R Filgueira1, Yuan Huang, Klaus Witt
1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Analytical Chemistry
|October 25, 2011
Summary
Flow splitters in online comprehensive two-dimensional liquid chromatography (LC × LC) enhance system flexibility. This method significantly boosts 2D peak capacity and observed peaks, enabling more efficient analyses.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Online comprehensive two-dimensional liquid chromatography (LC × LC) is a powerful separation technique.
- Flow splitters are common in 2D gas chromatography (GC × GC) but less explored in LC × LC.
Purpose of the Study:
- To investigate the impact of using flow splitters in online LC × LC.
- To assess the benefits of independent optimization of chromatographic dimensions.
Main Methods:
- Implementation of a post-first-dimension flow splitter in an LC × LC system.
- Comparison of system performance with and without a flow splitter.
Main Results:
- A 2-fold increase in corrected 2D peak capacity was observed.
- The number of observed peaks increased significantly within a 15-min analysis time.
- Independent optimization of the first dimension was achieved, improving gradient time and re-equilibration.
Conclusions:
- Flow splitters offer increased resolving power and system flexibility in LC × LC.
- This approach allows for near-independent optimization of both chromatographic dimensions.
- The benefits outweigh potential sensitivity loss, leading to enhanced analytical performance.
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