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Localization of the Locus Coeruleus in the Mouse Brain

Localization of the Locus Coeruleus in the Mouse Brain

Published on: March 7, 2019

Developmental changes in pacemaker currents in mouse locus coeruleus neurons.

Ramatis B de Oliveira¹, Fernanda S Gravina, Rebecca Lim

¹School of Biomedical Sciences and Pharmacy, University of Newcastle, University Drive, NSW 2308, Australia. ramatis.deoliveira@uon.edu.au

|October 25, 2011

Summary

Mouse locus coeruleus (LC) neurons show developmental changes in electrophysiological properties. Pacemaker currents and voltage fluctuations increase, influencing spontaneous firing in adult LC neurons.

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Area of Science:

Neuroscience
Developmental Biology
Electrophysiology

Background:

Locus coeruleus (LC) neurons are crucial for regulating arousal and stress.
Understanding the development of LC neuron electrophysiology is key to comprehending their function.

Purpose of the Study:

To compare electrophysiological properties and pacemaker currents in infant and adult mouse LC neurons.
To investigate developmental changes influencing LC neuron pacemaking and spontaneous firing.

Main Methods:

Whole-cell patch-clamp recordings in current-clamp and voltage-clamp modes.
Pharmacological manipulation using tetrodotoxin (TTX) and 4-aminopyridine (4-AP).

Main Results:

Pacemaker currents (TTX-sensitive Na(+) and voltage-dependent K(+)) increased with development.
Tetrodotoxin-resistant spikes and voltage fluctuations were more prominent in adult LC neurons.
Hyperpolarization-activated (I(H)) and Ca(2+) currents were not involved in pacemaking.

Conclusions:

Mouse LC neurons exhibit significant electrophysiological maturation during development.
Developmental changes in pacemaker currents and voltage fluctuations impact LC neuron pacemaking and spontaneous activity.