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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
06:23

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Published on: April 27, 2019

SUMO playing tag with ubiquitin.

Gerrit J K Praefcke1, Kay Hofmann, R Jürgen Dohmen

  • 1Center for Molecular Medicine Cologne, Institute for Genetics, University of Cologne, Cologne Biocenter, Zülpicher Straße 47a, D-50674 Cologne, Germany.

Trends in Biochemical Sciences
|October 25, 2011
PubMed
Summary
This summary is machine-generated.

Protein modifiers ubiquitin and small ubiquitin-related modifier (SUMO) share functional links, impacting DNA repair, cell growth, and disease. Their interplay is crucial for genome stability and therapeutic strategies.

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SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
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Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Ubiquitin and small ubiquitin-related modifier (SUMO) are structurally related protein modifiers.
  • These modifiers exhibit significant functional interrelations, including shared attachment sites and SUMO-dependent ubiquitylation.
  • Interactions between SUMO and ubiquitin are vital for numerous cellular processes.

Purpose of the Study:

  • To review recent findings on the intersections between SUMO and ubiquitin.
  • To highlight the influence of these interactions on genome stability and cell growth.
  • To discuss the relevance of SUMO-ubiquitin interplay in pathogen resistance and cancer treatment.

Main Methods:

  • Literature review of recent scientific findings.
  • Analysis of functional interrelations between protein modifiers.
  • Synthesis of data concerning genome stability, cell growth, and disease relevance.

Main Results:

  • SUMO and ubiquitin target the same sites in some substrates and exhibit SUMO-dependent ubiquitylation in others.
  • Sequential sumoylation and ubiquitylation of distinct substrates are required for cellular processes like DNA damage repair.
  • Enzymes controlling one modification can be regulated by the other, and some proteins promote both.

Conclusions:

  • The interplay between SUMO and ubiquitin significantly impacts genome stability and cell growth.
  • Understanding these interactions is crucial for developing strategies against pathogens and for cancer treatment.
  • Further research into SUMO-ubiquitin crosstalk promises advancements in medicine and biology.