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Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
Toxoplasma gondii: identification and immune response against a group of proteins involved in cellular invasion
Samira Azzouz1, Mimoun Maache, Antonio Osuna
1Department of Parasitology and Medical Mycology, Faculté de Pharmacie, Université de Lyon, Université Claude Bernard-Lyon I, ISPB, 8 Avenue Rockefeller, 69373 Lyon Cedex 08, France. samira.azzouz-maache@univ-lyon1.fr
Abstract:
Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites.
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