Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...
Influenza01:27

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long trace profiler downward- and sideways-facing measurement simulations.

The Review of scientific instruments·2026
Same author

Co-occurrence of the cephalosporinase cepA and carbapenemase cfiA genes in a Bacteroides fragilis division II strain, an unexpected finding.

The Journal of antimicrobial chemotherapy·2024
Same author

Domain wall propagation and pinning induced by current pulses in cylindrical modulated nanowires.

Nanoscale·2023
Same author

Evidence of Skyrmion-Tube Mediated Magnetization Reversal in Modulated Nanowires.

Materials (Basel, Switzerland)·2021
Same author

Multiresistance to Nonazole Fungicides in Aspergillus fumigatus TR<sub>34</sub>/L98H Azole-Resistant Isolates.

Antimicrobial agents and chemotherapy·2021
Same author

Is it possible to extrapolate the rates of resistance of Escherichia coli from asymptomatic bacteriuria in pregnant women to those of E. coli in uncomplicated community-acquired UTI?

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2019

Related Experiment Video

Updated: May 28, 2026

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Phosphorylation of the human respiratory syncytial virus N protein provokes a decrease in viral RNA synthesis.

A Asenjo1, I Cuesta, A Vivo

  • 1Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III (ISCIII), Crta. Majadahonda-Pozuelo km 2, Majadahonda, Madrid 28220, Spain.

Virus Research
|October 25, 2011
PubMed
Summary

Phosphorylation of the human respiratory syncytial virus (HRSV) N protein at Y38 regulates nucleocapsid (NC) template activity, impacting viral RNA synthesis. This finding offers insights into HRSV replication mechanisms.

More Related Videos

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

Related Experiment Videos

Last Updated: May 28, 2026

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
09:08

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Published on: July 27, 2021

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Human respiratory syncytial virus (HRSV) infection involves the N protein.
  • N protein phosphorylation at tyrosine Y38 is a regulated process during HRSV infection.

Purpose of the Study:

  • To investigate the impact of Y38 phosphorylation on HRSV N protein nucleocapsid (NC) template activity.
  • To understand how N protein modifications affect viral RNA synthesis.

Main Methods:

  • Site-directed mutagenesis of HRSV N protein tyrosine residues (Y38, Y23, Y69) to phenylalanine or aspartic acid.
  • Analysis of nucleocapsid formation and P protein interaction.
  • Assessment of minigenome-based viral transcription and replication.

Main Results:

  • N protein variants maintained NC formation and P protein interaction.
  • Altered NC template activity was observed in Y38 substituted variants.
  • Viral transcription and replication of HRSV minigenomes were distinctly affected.

Conclusions:

  • Y38 phosphorylation of the HRSV N protein modulates NC template activity.
  • This modulation likely occurs by altering interactions among monomeric NC components.
  • Understanding these mechanisms is crucial for HRSV replication studies.