Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Paracrine Signaling

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Stem Cell Therapy for Tissue Regeneration

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Elevated Plasma Nitrate Levels in Patients with Acute Coronary Syndrome.

The American journal of cardiology·2025

Same author

Nitric oxide-releasing gel accelerates healing in a diabetic murine splinted excisional wound model.

Frontiers in medicine·2023

Same author

Soluble Guanylate Cyclase β1 Subunit Represses Human Glioblastoma Growth.

Cancers·2023

Same author

"They're very passionate about making sure that women stay healthy": a qualitative examination of women's experiences participating in a community paramedicine program.

BMC health services research·2021

Same author

Discovery of Some of the Biological Effects of Nitric Oxide and Its Role in Cell Signaling (Nobel Lecture).

Angewandte Chemie (International ed. in English)·2021

Same author

John Mendelsohn: A visionary scientist, oncologist and leader.

Genes & cancer·2019

Search research articles

Related Experiment Video

Updated: May 28, 2026

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

Nitric oxide-cyclic GMP signaling in stem cell differentiation.

Kalpana Mujoo¹, Joshua S Krumenacker, Ferid Murad

¹Brown Foundation Institute of Molecular Medicine, Texas Therapeutics Institute, University of Texas Health Science Center at Houston, Houston, TX 77030, USA. Kalpana.Mujoo@uth.tmc.edu

Free Radical Biology & Medicine

|October 25, 2011

Summary

The nitric oxide-cyclic GMP (NO-cGMP) pathway is crucial for stem cell differentiation into various cell types, including heart cells. This pathway shows promise for stem cell-based therapies.

More Related Videos

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Related Experiment Videos

Last Updated: May 28, 2026

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Area of Science:

Physiology
Cell Biology
Developmental Biology

Background:

The nitric oxide-cyclic GMP (NO-cGMP) pathway regulates vital physiological functions in cardiovascular and nervous systems.
NO also controls cellular processes like growth, survival, apoptosis, proliferation, and differentiation.
The role of the NO-cGMP pathway in development and differentiation has been explored in embryos and stem cells.

Purpose of the Study:

To review the role of the NO-cGMP pathway in stem cell differentiation.
To highlight its significance in differentiating stem cells into specific lineages, particularly myocardial cells.
To discuss the implications for stem cell-based therapy.

Main Methods:

Pharmacological manipulation of the NO-cGMP pathway using activators and inhibitors.
Genetic manipulation of NO signaling components.
Review of existing studies on NO-cGMP in embryonic and stem cell differentiation.

Main Results:

Evidence suggests the NO-cGMP pathway is involved in regulating stem cell differentiation.
Studies indicate this pathway's importance in the development of various cell lineages.
Specific focus on differentiation into myocardial cells.

Conclusions:

The NO-cGMP pathway plays a significant role in stem cell differentiation.
This pathway is a key regulator in the development of specialized cell types.
Understanding NO-cGMP signaling is critical for advancing stem cell-based therapies.