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Related Experiment Video

Updated: May 28, 2026

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
09:07

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Published on: February 5, 2018

Setting a highway for converting skin into neurons.

Vania Broccoli1, Massimiliano Caiazzo, Maria Teresa Dell'Anno

  • 1San Raffaele Scientific Institute, Via Olgettina 58, Milan 20132, Italy. broccoli.vania@hsr.it

Journal of Molecular Cell Biology
|October 25, 2011
PubMed
Summary

Human skin fibroblasts can be directly converted into induced neuronal (iN) cells using transcription factors and microRNAs. This breakthrough enables efficient in vitro generation of functional human neurons for disease modeling and brain repair.

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Area of Science:

  • Stem cell biology
  • Neuroscience
  • Molecular biology

Background:

  • Induced neuronal cells offer a promising avenue for studying neurological disorders.
  • Generating functional human neurons in vitro is crucial for disease modeling and regenerative medicine.

Purpose of the Study:

  • To investigate the direct conversion of human skin fibroblasts into induced neuronal (iN) cells.
  • To explore the efficacy of transcription factors and microRNAs in this direct conversion process.

Main Methods:

  • Utilizing specific combinations of transcription factors.
  • Employing microRNAs to enhance the reprogramming process.
  • Direct conversion of human skin fibroblasts into iN cells.

Main Results:

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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

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Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
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Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

Published on: May 1, 2019

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Last Updated: May 28, 2026

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
09:07

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts

Published on: February 5, 2018

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors
07:33

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

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  • Successful direct conversion of human fibroblasts into iN cells was achieved.
  • The generated iN cells possess elaborated functional properties.

Conclusions:

  • This method provides a straightforward and efficient way to generate human neurons in vitro.
  • These findings support the use of iN cells for disease modeling and cell-based brain repair strategies.