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Methylated DNA Immunoprecipitation

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

The MethDet: a technology for biomarker development.

Victor V Levenson¹, Anatoliy A Melnikov

¹Department of Radiation Oncology, Rush University Medical Center, Chicago, IL 60612, USA. victor_levenson@rush.edu

Expert Review of Molecular Diagnostics

|October 26, 2011

Summary

This study introduces a novel platform technology for genome-wide DNA methylation analysis using minimal samples. This methylation detection (MethDet) technology enables early disease biomarker discovery and monitoring.

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Methodology for Accurate Detection of Mitochondrial DNA Methylation

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

Area of Science:

Epigenetics
Molecular Biology
Biomarker Discovery

Background:

Early disease detection relies on biomarkers, necessitating robust development and validation methods.
Current methods may require substantial sample volumes, limiting accessibility for certain sample types.

Purpose of the Study:

To present a platform technology for genome-wide DNA methylation analysis from small sample volumes.
To enable early disease detection and monitoring through epigenetic profiling.

Main Methods:

Developed a methylation detection (MethDet) platform for genome-wide DNA methylation analysis.
Utilized small sample inputs: biopsy, formalin-fixed paraffin-embedded tissue sections, or 0.4 ml plasma.
Enabled genome-wide association studies comparable to single-nucleotide polymorphism analysis.

Main Results:

MethDet technology analyzes epigenetic changes, reflecting disease-dependent alterations in gene expression.
The assay effectively uses highly fragmented DNA, including cell-free circulating DNA from plasma.
Identified disease-specific changes, treatment effects, and disease activity variations.

Conclusions:

MethDet offers a powerful tool for genome-wide epigenetic analysis with minimal sample requirements.
This technology facilitates the discovery and validation of novel biomarkers for early disease detection.
MethDet can track disease progression, treatment response, and activity through epigenetic modifications.