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Updated: May 28, 2026
Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment
Published on: June 2, 2023
The effect of hepatectomy on plasma lipolytic enzyme activity and circulating lipid levels: an experimental study on
Sophocles Lanitis1, Evangelos Lolis, Nikolaos Dafnios
12nd Surgical Department, Red Cross Athens General Hospital, Athens, Greece. drlanitis@yahoo.com
Background/Aims:
The aim of this experimental study was to investigate the effect of liver resection and resulting transient hepatic steatosis on circulating lipid levels and plasma lipolytic enzyme activity.
Methodology:
Forty-eight male Wistar rats were assigned to the sham (Group A=21) or the hepatectomy group (Group B=27) and were further subdivided according to the day they were sacrificed (POD 1, 3 and 7). We measured hepatic lipase (HL) and total lipase (TL) activity and plasma levels of AST, ALT, triglycerides, HDL and cholesterol.
Results:
There were no significant differences in the activities of HL and TL between the groups. Nevertheless, significant differences were observed in the levels of HDL (p=0.001) and cholesterol (p=0.001) on the 1st POD, and in triglycerides (p=0.045) on the 7th POD. A significant drop in the levels of HDL and cholesterol on the 1st POD was followed by a rapid recovery (3rd POD) and maintenance of these levels in group B until the 7th POD, while high levels of triglycerides (TG) were observed only on POD 7.
Conclusions:
After hepatectomy the activities of the lipolytic enzymes recover early and remain stable during regeneration. HDL and cholesterol levels are reversely proportional to the liver stress.
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