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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: May 28, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Activity-based protein profiling for natural product target discovery.

Joanna Krysiak1, Rolf Breinbauer

  • 1Institute of Organic Chemistry, Graz University of Technology, Austria.

Topics in Current Chemistry
|October 26, 2011
PubMed
Summary

Activity-based protein profiling (ABPP) is a powerful technique for identifying natural product drug targets. This method utilizes natural products

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Pharmacology

Background:

  • Natural products are a rich source of biologically active molecules and drug candidates.
  • Identifying the specific protein targets of natural products is crucial for drug development.
  • Novel techniques are needed to efficiently annotate these biological targets.

Purpose of the Study:

  • To review the current status and applications of activity-based protein profiling (ABPP) for natural product target identification.
  • To highlight the utility of ABPP in annotating targets for natural products with various electrophilic functional groups.

Main Methods:

  • Exploiting protein-reactive functional groups present in natural products.
  • Utilizing activity-based protein profiling (ABPP) as a key technique.

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Published on: August 6, 2018

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Last Updated: May 28, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

  • Developing probe molecules from biomimetic natural product libraries.

    • Main Results:

    • ABPP offers significant opportunities for identifying natural product targets.
    • Successful annotation of biological targets for natural products containing epoxides, lactones, lactams, and Michael acceptors.
    • Demonstration of probe development from biomimetic natural product libraries.

    Conclusions:

    • Activity-based protein profiling is a valuable tool for elucidating natural product mechanisms of action.
    • This approach facilitates the discovery of new drug candidates from natural product sources.
    • Further development of ABPP and biomimetic probes will advance natural product drug discovery.