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p53 regulates the expression of human angiotensin II AT(2) receptor interacting protein (ATIP1) gene

Zujian Chen1, Xiqiang Liu, Cheng Wang

  • 1Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA.

Oncology Letters
|October 26, 2011
PubMed

Insights

The tumor suppressor Angiotensin II AT(2) receptor interacting protein 1 (ATIP1) is regulated by the p53 protein. p53 directly binds to the ATIP1 promoter, influencing its expression and impacting cancer progression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Angiotensin II AT(2) receptor interacting protein 1 (ATIP1) is recognized as a tumor suppressor.
  • Understanding the regulatory mechanisms of ATIP1 is crucial for cancer research.

Purpose of the Study:

  • To investigate the transcriptional regulation of the human ATIP1 gene.
  • To determine the role of the p53 protein in controlling ATIP1 expression.

Main Methods:

  • Cloning and promoter activity assay of the 5' flanking region of the ATIP1 gene.
  • Identification of p53 binding sites within the ATIP1 promoter.
  • Reporter gene assays and chromatin-immunoprecipitation to confirm p53-ATIP1 promoter interaction.
  • p53 knockdown and mutation studies to assess functional impact.

Main Results:

  • A 2.2 kb fragment of the ATIP1 5' flanking region demonstrated promoter activity.
  • Two putative p53 binding sites were identified in the minimal ATIP1 promoter.
  • p53 significantly enhanced ATIP1 expression, while p53 knockdown reduced it.
  • Direct binding of p53 to the ATIP1 promoter was confirmed, and mutation of these sites abolished p53's regulatory effect.

Conclusions:

  • The ATIP1 gene is transcriptionally regulated by the p53 protein.
  • p53 plays a significant role in modulating ATIP1 expression.
  • ATIP1, regulated by p53, may be important in cancer initiation and progression.

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