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Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

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Magnetic core-shell fluorescent pH ratiometric nanosensor using a Stöber coating method.

A Lapresta-Fernández¹, T Doussineau, A J Moro

¹Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Jena, Germany. lapresta@ugr.es

Analytica Chimica Acta

|October 27, 2011

Summary

We developed magnetic fluorescent sensor nanoparticles for ratiometric pH measurements between pH 5-8. These dual-dye doped silica-coated maghemite nanoparticles offer reliable pH sensing with potential applications in various sample matrices.

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Area of Science:

Nanotechnology
Materials Science
Analytical Chemistry

Background:

Development of novel sensor nanoparticles is crucial for advanced analytical applications.
Magnetic nanoparticles offer unique advantages for separation and concentration.
Ratiometric sensing provides enhanced accuracy and stability.

Purpose of the Study:

To synthesize and characterize magnetic fluorescent sensor nanoparticles for ratiometric pH measurements.
To embed two distinct fluorophores within silica shells on maghemite cores.
To evaluate the performance of these nanoparticles in the pH range of 5-8.

Main Methods:

Modified Stöber method for silica coating of maghemite (γ-Fe(2)O(3)) nanocrystals.
Incorporation of sulforhodamine B (reference dye) and fluorescein (pH-sensitive dye) into silica shells.
Characterization using dynamic light scattering (DLS), transmission electron microscopy (TEM), X-ray diffraction (XRD), and vibrating sample magnetometry (VSM).

Main Results:

Uniformly sized (50-70nm) silica-coated maghemite nanoparticles were successfully synthesized.
Nanoparticles exhibited characteristic maghemite spinel structure and satisfactory magnetic properties for separation.
Ratiometric fluorescence intensity correlated with pH changes in the 5-8 range, with rapid response times.
The sensing method showed no significant statistical difference compared to a reference procedure in complex matrices.

Conclusions:

The developed magnetic fluorescent sensor nanoparticles are effective for ratiometric pH sensing.
The silica coating provides a stable platform for embedding dyes and maintaining nanoparticle integrity.
The method is robust and statistically validated for use in water, buffer, and serum-containing samples.