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Related Experiment Videos

Carbohydrate-enzyme conjugates for competitive EIA.

E Vorberg1, D R Bundle

  • 1Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.

Journal of Immunological Methods
|August 28, 1990
PubMed
Summary
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Enzyme-saccharide conjugates offer a robust method for quantifying bacterial polysaccharide antigens and measuring the inhibitory power of oligosaccharides. This direct, competitive enzyme immunoassay format simplifies the determination of binding constants and relative inhibition.

Area of Science:

  • Biochemistry
  • Immunology
  • Carbohydrate Chemistry

Background:

  • Enzyme glycoconjugates are valuable reagents in immunoassays.
  • Direct, competitive enzyme immunoassays require efficient and durable reagents.
  • Quantifying bacterial polysaccharide antigens and oligosaccharide inhibitory power is crucial.

Purpose of the Study:

  • To develop and optimize enzyme-saccharide conjugates for direct, competitive enzyme immunoassays.
  • To establish protocols for measuring association constants and relative inhibitory power of oligosaccharides.
  • To enable sensitive quantitative assays for bacterial polysaccharide antigens.

Main Methods:

  • Preparation of enzyme-saccharide conjugates using established glycoconjugate methodologies.
  • Optimization of assay protocols by investigating antibody and antigen-enzyme conjugate concentrations.

Related Experiment Videos

  • Study of four monoclonal antibody-carbohydrate antigen systems.

    • Main Results:

    • Enzyme-saccharide conjugates demonstrated efficiency and durability as reagents.
    • The assay format facilitated simple measurement of association constants and relative inhibitory power of oligosaccharides.
    • Sensitive quantitative assays for bacterial polysaccharide antigens were achieved.

    Conclusions:

    • Enzyme-saccharide conjugates are effective reagents for direct, competitive enzyme immunoassays.
    • The developed assay format allows for convenient measurement of oligosaccharide inhibitory power and extrapolation of association constants.
    • This methodology provides a sensitive tool for bacterial antigen quantification.