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Updated: May 28, 2026
Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
Published on: June 23, 2014
[Establishment of transgenic mice with visualized neovascularization]
Zhen-lin Li1, Yi Gao, Jun-shuang Jia
1Department of Histology and Embryology, Southern Medical University, Guangzhou, China. zhenlin@fimmu.com
Objective:
To establish transgenic mice with GFP expression in the vascular endothelium during neovascularization.
Methods:
The vector nestin-hsp68-gfp containing nestin second intron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry.
Results:
Thirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth.
Conclusions:
The transgenic mice with GFP expression in the vascular endothelium during neovascularization have been successfully established.
