Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus

A restriction fragment library which covered the entire genome of Bombyx mori nuclear polyhedrosis virus (BmNPV) was constructed using plasmid vectors. By analysis of cloned and viral DNA by double digestion with endonucleases and by hybridization techniques, a complete physical map of BmNPV was constructed for BamHI, EcoRI, HindIII, KpnI, PstI and SmaI. Five regions of repeated sequences containing EcoRI sites were also found and mapped.