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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Related Experiment Video

Updated: May 28, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Photoaffinity labeling in activity-based protein profiling.

Paul P Geurink1, Laurette M Prely, Gijs A van der Marel

  • 1Leiden Institute of Chemistry and the Netherlands Proteomics Centre, Leiden University, The Netherlands.

Topics in Current Chemistry
|October 27, 2011
PubMed
Summary
This summary is machine-generated.

Photo-activatable probes are revolutionizing enzyme activity studies. These chemical biology tools help identify enzyme functions, locations, and drug effects by forming covalent bonds with target enzymes.

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Last Updated: May 28, 2026

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Area of Science:

  • Chemical Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Activity-based protein profiling (ABPP) is a key method for investigating enzyme function in native biological systems.
  • ABPP utilizes tagged substrate analogs that covalently bind to enzyme active sites, enabling enzyme discovery and characterization.
  • Photo-activatable probes represent a specialized class of ABPP tools, particularly useful for enzymes lacking backbone catalytic nucleophiles.

Purpose of the Study:

  • To provide a comprehensive overview of photo-activatable groups used in activity-based protein profiling.
  • To detail the photochemistry and diverse research applications of these probes.
  • To compare the efficacy of various photo-activatable groups in chemical biology studies.

Main Methods:

  • Review of literature on photo-activatable groups for ABPP.
  • Analysis of probe chemistry, including photoactivation mechanisms.
  • Discussion of comparative studies evaluating probe efficiency and applications.

Main Results:

  • Identification and categorization of various photo-activatable groups available for ABPP.
  • Elucidation of the photochemical properties and reaction mechanisms of these groups.
  • Presentation of comparative data on the performance of different photo-activatable probes across various research contexts.

Conclusions:

  • Photo-activatable probes are versatile tools in chemical biology for studying enzyme activity.
  • Understanding the photochemistry of these probes is crucial for their effective application.
  • Comparative studies highlight the strengths and weaknesses of different photo-activatable groups, guiding future probe design.