Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circulating PEG-indoleamine 2,3-dioxygenase ameliorates diverse inflammatory diseases without toxicity or compromising immunocompetence.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Planar neural organoids as an advanced in vitro model to study patient-derived melanoma metastasis.

Scientific reports·2026
Same author

Correction: Injectable hydrogel-based localized delivery of IDO-galectin-3 mitigates neuroinflammation and promotes neuronal sparing after spinal cord contusion in rats.

Journal of materials chemistry. B·2026
Same author

Plasmid DNA vaccines encapsulated in lipid nanoparticles elicit STING-dependent type 1 interferon release.

Molecular therapy. Advances·2026
Same author

A single intra-articular injection of IDO-Gal3 shifts synovial fluid metabolic profiles for up to six weeks in male rats with knee instability.

Biochimie·2026
Same author

Injectable hydrogel-based localized delivery of IDO-galectin-3 mitigates neuroinflammation and promotes neuronal sparing after spinal cord contusion in rats.

Journal of materials chemistry. B·2026

Related Experiment Video

Updated: May 28, 2026

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

Surfaces that sequester serum-borne heparin amplify growth factor activity.

Gregory A Hudalla1, Justin T Koepsel, William L Murphy

  • 1Department of Biomedical Engineering, University of Wisconsin-Madison, 53705, USA.

Advanced Materials (Deerfield Beach, Fla.)
|October 27, 2011
PubMed
Summary

Surfaces with heparin-binding peptides capture heparin from serum. This localized heparin then concentrates growth factors, boosting their bioactivity at the cell-material interface.

More Related Videos

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

Related Experiment Videos

Last Updated: May 28, 2026

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
12:27

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Biochemistry

Background:

  • Cell culture supplements like fetal bovine serum contain heparin.
  • Heparin plays a role in growth factor localization and bioactivity.
  • Cell-material interfaces are critical for understanding cellular responses.

Purpose of the Study:

  • To investigate the non-covalent sequestration of heparin by surfaces presenting heparin-binding peptides.
  • To determine if sequestered heparin can localize growth factors.
  • To assess the impact on growth factor bioactivity at the cell-material interface.

Main Methods:

  • Utilizing surfaces functionalized with heparin-binding peptides.
  • Incubating surfaces in culture media containing fetal bovine serum to allow heparin sequestration.
  • Analyzing the localization of heparin and associated growth factors at the cell-material interface using appropriate assays.
  • Measuring growth factor bioactivity.

Main Results:

  • Heparin-binding peptide surfaces successfully sequestered heparin from culture supplements.
  • Sequestered heparin localized serum-borne growth factors to the cell-material interface.
  • This localized concentration of growth factors resulted in amplified bioactivity.

Conclusions:

  • Surfaces engineered with heparin-binding peptides can effectively capture heparin.
  • This mechanism concentrates growth factors, enhancing their bioactivity.
  • This strategy offers a novel approach for controlling cell behavior through biomaterial design.