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Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Candidiasis01:20

Candidiasis

Candidiasis is a fungal infection caused by opportunistic species of Candida. It can affect various anatomical sites, including the skin, oral cavity, nails, and genitourinary tract. Among its forms, vaginal candidiasis is the most common type of mucosal infection. It typically results from the overgrowth of Candida albicans in the vaginal mucosa. Under normal conditions, C. albicans exists as a commensal organism within the vaginal microbiota, regulated by the dominance of lactobacilli, which...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
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Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis

Published on: June 13, 2021

Ribosomal RNA processing in Candida albicans.

Michael L Pendrak1, David D Roberts

  • 1Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. mpendrak@helix.nih.gov

RNA (New York, N.Y.)
|October 27, 2011
PubMed
Summary
This summary is machine-generated.

Candida albicans rRNA processing shows distinct mechanisms. The A0-A1 fragment aids proliferation assessment, and novel pathways regulate cotranscriptional versus post-transcriptional maturation.

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Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis

Published on: March 19, 2019

Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • Ribosome assembly is a fundamental process involving rRNA precursor conversion.
  • Candida albicans, an important human pathogen, exhibits unique rRNA processing characteristics.
  • Understanding rRNA maturation is crucial for cellular function and proliferation studies.

Purpose of the Study:

  • To elucidate the mechanisms of rRNA processing in Candida albicans.
  • To identify novel regulatory pathways controlling rRNA maturation.
  • To investigate the role of specific rRNA precursors in cellular physiology.

Main Methods:

  • Analysis of rRNA processing sites (A0, A1, A2, A3) in Candida albicans.
  • Characterization of rRNA precursor species during different growth phases.
  • Comparison of rRNA processing with Saccharomyces cerevisiae and other eukaryotes.
  • Investigation of U3 snoRNA-dependent processing during proliferation.

Main Results:

  • Identified a stable A0-A1 rRNA fragment useful for proliferation rate assessment.
  • Observed differences in 5.8S rRNA maturation compared to Saccharomyces cerevisiae.
  • Demonstrated structural similarities in external and internal transcribed spacers despite low sequence identity.
  • Found evidence for both cotranscriptional and post-transcriptional rRNA processing, with U3 snoRNA involvement in rapid growth.
  • Characterized a 23S pre-rRNA species accumulating in stationary phase, responsive to nutrient availability.

Conclusions:

  • Candida albicans employs distinct rRNA processing mechanisms compared to other eukaryotes.
  • The A0-A1 fragment serves as a reliable marker for Candida albicans proliferation.
  • New regulatory mechanisms governing the balance between cotranscriptional and post-transcriptional rRNA processing are revealed in Candida albicans.
  • These findings contribute to a deeper understanding of ribosome biogenesis and its regulation in fungi.