Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Long non-coding RNA triplex-dependent regulation of melanoma gene networks.

NAR genomics and bioinformatics·2026

Same author

Identifying the drivers of genetic risk in patients with multiple primary melanoma.

The Journal of investigative dermatology·2026

Same author

Decoy antibodies block extracellular HSP70, prevent self-signaling, and inhibit melanoma cell survival.

Cell reports·2026

Same author

Multilevel metabolic adaptation to exercise training.

Communications medicine·2026

Same author

Nuclear import of malaria RNA rewires splicing in host immune cells.

Cell reports·2026

Same author

Identification of MITF-regulated transcript isoforms of ubiquitously expressed genes.

The Journal of investigative dermatology·2026

Search research articles

Related Experiment Video

Updated: May 28, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Feed-forward microprocessing and splicing activities at a microRNA-containing intron.

Maja M Janas¹, Mehdi Khaled, Steffen Schubert

¹Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA.

|October 27, 2011

Summary

This summary is machine-generated.

The study reveals that microRNA (miRNA) processing and messenger RNA (mRNA) splicing are linked. Spliceosome assembly on an intron helps process the intronic miRNA, demonstrating a cooperative mechanism.

More Related Videos

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Related Experiment Videos

Last Updated: May 28, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Most mammalian microRNA (miRNA) genes are located within introns of other genes.
The mechanisms coordinating the processing of primary transcripts into mature miRNAs and spliced mRNAs are not well understood.

Purpose of the Study:

To investigate the interplay between microRNA processing and mRNA splicing at an intronic miRNA locus.
To elucidate the molecular mechanisms coordinating the biogenesis of intronic microRNAs and the splicing of host gene transcripts.

Main Methods:

Analysis of miR-211 processing and melastatin splicing in relation to Drosha activity.
Site-directed mutagenesis of intron 6 splice sites, branch point, and polypyrimidine tract.
Knockdown of U1 splicing factors to assess global impact on intronic miRNA expression.

Main Results:

Microprocessing of intronic miR-211 promotes the splicing of the melastatin exon 6-exon 7 junction, requiring Drosha RNase III activity.
Mutations in the 5' splice site (5'SS) of intron 6 impaired miR-211 biogenesis and Drosha recruitment, indicating 5'SS recognition is crucial.
Knockdown of U1 splicing factors led to reduced intronic miRNA expression, supporting a global role for splicing factors.

Conclusions:

Demonstrates a novel, mutually cooperative relationship between microprocessing of intronic miRNAs and mRNA splicing.
Suggests that the initiation of spliceosome assembly on an intron may promote the microprocessing of intronic miRNAs.