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Published on: June 21, 2014
Efficient generation of iPS cells from skeletal muscle stem cells
Kah Yong Tan1, Sarah Eminli, Simone Hettmer
1Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, Massachusetts, United Stated of America.
Plos One
|October 27, 2011
Summary
Adult stem cells reprogram more efficiently than differentiated cells, challenging the idea that epigenetic memory impacts induced pluripotent stem cell differentiation potential in skeletal muscle.
Area of Science:
- Cell biology
- Stem cell research
- Epigenetics
Background:
- Somatic cell reprogramming into induced pluripotent stem cells (iPSCs) is often inefficient.
- The differentiation status of somatic cells may affect reprogramming efficiency and iPSC differentiation potential.
- Understanding factors limiting reprogramming efficiency is crucial for advancing regenerative medicine.
Purpose of the Study:
- To investigate how lineage commitment influences iPSC derivation efficiency and differentiation potential.
- To compare reprogramming efficiency between adult stem cells and mature cells in skeletal muscle.
- To determine if "epigenetic memory" biases iPSC differentiation from different somatic lineages.
Main Methods:
- Utilized clonal assays and a second-generation inducible reprogramming system.
- Evaluated reprogramming in adult stem cell and mature cell populations within mouse skeletal muscle.
- Assessed iPSC derivation efficiency and differentiation potential from various cell types.
Main Results:
- Adult stem cells (satellite cells, mesenchymal progenitors) reprogrammed with significantly higher efficiency than differentiated cells (myoblasts, fibroblasts).
- No evidence of biased differentiation potential was found in iPSCs derived from myogenically committed cells, contrary to some previous reports.
- Reprogramming efficiency is strongly influenced by the cell's lineage commitment status.
Conclusions:
- Adult stem cells are more efficiently reprogrammed into iPSCs compared to terminally differentiated cells.
- The concept of "epigenetic memory" significantly influencing iPSC differentiation potential in skeletal muscle was not supported by these findings.
- Cellular differentiation status is a key determinant of reprogramming efficiency, but not necessarily of subsequent iPSC differentiation potential.
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