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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: May 28, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.

Gregory Marusov¹, Andrew Sweatt, Kathryn Pietrosimone

¹Department of Molecular and Cell Biology, The University of Connecticut, 91 North Eagleville Road, Storrs, Connecticut 06269-3125, United States.

Environmental Science & Technology

|October 28, 2011

Summary

This summary is machine-generated.

Grating-coupled surface plasmon resonance imaging (GCSPRI) offers a rapid, label-free method for detecting multiple toxins and pathogens simultaneously. This highly multiplexed assay uses disposable biosensor chips for cost-effective, large-scale diagnostics.

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

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Last Updated: May 28, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Area of Science:

Biosensing and diagnostics
Surface plasmon resonance
Optical imaging technologies

Background:

Grating-coupled surface plasmon resonance imaging (GCSPRI) uses a diffraction grating on a gold-coated chip to couple light into surface plasmons.
Analyte capture at the chip surface alters the coupling angle, enabling sensitive detection.
Disposable, mass-producible biosensor chips in microarray format enhance multiplexing capabilities.

Purpose of the Study:

To demonstrate the utility of GCSPRI for direct, real-time detection of diverse analytes.
To showcase GCSPRI's capability for simultaneous, large-scale, and label-free sensing.
To validate GCSPRI for diagnostic and environmental monitoring applications.

Main Methods:

Utilized GCSPRI instrumentation capable of measuring over 1000 discrete regions of interest (ROIs) simultaneously.
Employed antibody microarrays immobilized on disposable sensor chips for capture molecule immobilization.
Applied GCSPRI to detect various agents including protein toxins, bacterial cells, and viruses.

Main Results:

Successfully detected multiple analytes in near real-time, including a nontoxic form of Pseudomonas aeruginosa exotoxin A (ntPE), Bacillus globigii, Mycoplasma hyopneumoniae, Listeria monocytogenes, Escherichia coli, and M13 bacteriophage.
Demonstrated a large dynamic range for analyte detection.
Achieved simultaneous assessment of toxins, pathogens, and specific antibodies.

Conclusions:

GCSPRI is a versatile, label-free technology for rapid, highly multiplexed detection of diverse biological agents.
The system enables simultaneous quantification of multiple analytes using minimal sample and reagent volumes.
GCSPRI shows significant potential for diagnostic and environmental sensing applications.