Functional photoacoustic microscopy of pH
Related Experiment Video
Updated: May 28, 2026
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Muhammad Rameez Chatni1, Junjie Yao, Amos Danielli
1Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130, USA.
Abstract:
pH is a tightly regulated indicator of metabolic activity. In mammalian systems, an imbalance of pH regulation may result from or result in serious illness. In this paper, we report photoacoustic microscopy (PAM) of a commercially available pH-sensitive fluorescent dye (SNARF-5F carboxylic acid) in tissue phantoms. We demonstrated that PAM is capable of pH imaging in absolute values at tissue depths of up to 2.0 mm, greater than possible with other forms of optical microscopy.
