Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-shot polarization digital holographic microscopy with phase bias equalization for full-field Jones matrix imaging.

Biomedical optics express·2026
Same author

Nonlinear Reconstruction of Images from Patterns Generated by Deterministic or Random Optical Masks-Concepts and Review of Research.

Journal of imaging·2022
Same author

Roadmap on Digital Holography-Based Quantitative Phase Imaging.

Journal of imaging·2021
Same author

Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2016
Same author

Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.

Neurophotonics·2015
Same author

Off-axis digital holographic camera for quantitative phase microscopy.

Biomedical optics express·2014
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: May 28, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Beyond the lateral resolution limit by phase imaging.

Yann Cotte1, M Fatih Toy, Christian Depeursinge

  • 1Ecole Polytechnique Fédérale de Lausanne (EPFL), Microvision and Microdiagnostics Group (MVD), Lausanne, 1015, Switzerland. yann.cotte@a3.epfl.ch

Journal of Biomedical Optics
|October 28, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a novel phase imaging technique to surpass the classical Abbe resolution limit. By manipulating illumination phase, researchers achieved higher accuracy in measuring distances, potentially overcoming fundamental limits in microscopy.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Related Experiment Videos

Last Updated: May 28, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Area of Science:

  • Optics
  • Microscopy
  • Image Processing

Background:

  • The classical Abbe resolution limit restricts the detail observable in optical microscopy.
  • Existing intensity imaging methods have inherent limitations in resolving fine structures.

Purpose of the Study:

  • To theoretically extend the Abbe resolution limit using phase imaging.
  • To experimentally demonstrate enhanced resolution capabilities through phase manipulation.

Main Methods:

  • Developing a theory for phase imaging with spatially varying illumination.
  • Utilizing high numerical aperture (NA = 0.93) digital holographic microscopy.
  • Employing angular scanning and phase visualization of point scatterers' interference.

Main Results:

  • Demonstrated enhanced bandpass information content with varied illumination phases.
  • Achieved proof-of-principle measurements using sub-wavelength nanometric holes.
  • Rebuilt Rayleigh's classical two-point resolution condition.

Conclusions:

  • The proposed phase imaging theory offers a pathway beyond classical resolution limits.
  • Experimental validation confirms the potential for enhanced spatial resolution, limited by signal-to-noise ratio.