Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Microneedling with adipose MSC exosomes versus monotherapy for androgenetic alopecia.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026

Same author

Cutaneous and serological profiles of idiopathic inflammatory myopathies in a hispanic population.

Reumatologia clinica·2026

Same author

From consensus to continuity: Redefining long-term melasma management.

Journal of the European Academy of Dermatology and Venereology : JEADV·2026

Same author

Correction: Reyes-Fernández et al. Comparative Regenerative Efficacy of PRP Combined with Chondrocytes or Mesenchymal Stem Cells for Intervertebral Disc Regeneration in a Rabbit Model. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 10007.

International journal of molecular sciences·2026

Same author

Clinical Images: Blistering targetoid skin lesions in a patient with rheumatoid arthritis.

Arthritis & rheumatology (Hoboken, N.J.)·2026

Same author

A Dermoscopic Great Imitator: Primary Cutaneous Coccidioidomycosis.

International journal of dermatology·2025

Search research articles

Related Experiment Video

Updated: May 28, 2026

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Published on: November 5, 2019

Decrease of virulence for BALB/c mice produced by continuous subculturing of Nocardia brasiliensis.

Janeth A Almaguer-Chávez¹, Oliverio Welsh, Hector G Lozano-Garza

¹Servicio de Dermatología, Hospital Universitario José E, González, Monterrey, N,L,, 64460 México.

BMC Infectious Diseases

|October 28, 2011

Summary

Continuous subculturing of Nocardia brasiliensis significantly reduced its virulence in mice. Attenuated Nocardia brasiliensis (T130) induced partial resistance against infection with the virulent strain.

More Related Videos

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

Related Experiment Videos

Last Updated: May 28, 2026

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Published on: November 5, 2019

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

Area of Science:

Medical Microbiology
Immunology
Infectious Diseases

Background:

Subculturing is a common method for attenuating human pathogens.
The virulence of Nocardia brasiliensis HUJEG-1 was investigated through continuous subculturing.

Purpose of the Study:

To evaluate the impact of extensive in vitro subculturing on the virulence of Nocardia brasiliensis HUJEG-1.
To assess the potential of attenuated Nocardia brasiliensis as a vaccine candidate.

Main Methods:

Nocardia brasiliensis HUJEG-1 was subcultured up to 130 times.
BALB/c mice were inoculated with different subculture generations (T0, T40, T80, T100, T130).
Immunological responses (CD4, CD8, CD14) were analyzed in infected footpads.

Main Results:

Subculturing reduced lesion formation and mycetoma development in mice.
The T130 generation showed a significant decrease in virulence, with 0% lesion incidence.
Immunization with T130 conferred partial resistance against challenge with the virulent T0 strain.
Immunostaining revealed CD4+ lymphocytic and macrophage infiltration in healing lesions.

Conclusions:

130 in vitro passages of N. brasiliensis HUJEG-1 led to a substantial reduction in virulence.
Attenuated N. brasiliensis cells can induce a state of partial resistance to infection in mice.