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Multi-layered plasma-polymerized chips for SPR-based detection.

Ram P Gandhiraman¹, Nam Cao Hoai Le, Chandra K Dixit

¹Biomedical Diagnostics Institute (BDI), Dublin City University, Glasnevin, Dublin-9, Ireland. ramprasad.gandhiraman@dcu.ie

ACS Applied Materials & Interfaces

|October 28, 2011

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A novel plasma polymerization technique enhances gold surface functionalization for biomolecular detection. This method significantly boosts carboxylic acid groups, improving biomolecule loading and assay performance.

Area of Science:

Materials Science
Biotechnology
Surface Chemistry

Background:

Surface functionalization is key for surface plasmon resonance (SPR) biosensors.
Coatings must maintain biomolecule activity and optimize loading for sensitive detection.

Purpose of the Study:

To develop a rapid, room-temperature method for functionalizing gold surfaces.
To compare siloxane/acrylic acid coatings with thiol-based coatings for SPR applications.
To demonstrate the efficacy of the functionalized surfaces in biomolecular assays.

Main Methods:

Gas-phase plasma polymerization for one-step surface functionalization.
Sequential deposition of siloxane (adhesion) and acrylic acid (functional) layers.
Secondary ion mass spectroscopy (SIMS) for compositional analysis.

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Surface plasmon enhanced ellipsometry and Biacore 3000 SPR for assay demonstration.

Main Results:

A 7-fold increase in carboxylic functionality compared to thiol precursors.
Ion-induced mixing confirmed by SIMS, creating a graded surface composition.
Successful DNA hybridization and protein immunoassay demonstrated on functionalized gold substrates.

Conclusions:

The siloxane/acrylic acid plasma polymerization offers a superior method for gold surface functionalization.
This technique enhances biomolecular detection capabilities in SPR systems.
The process is efficient, versatile, and applicable to various substrates for biosensing.