Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relationship between lipase and esterase.

T Tsujita1, K Shirai, Y Saito

  • 1Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.

Progress in Clinical and Biological Research
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Japanese single-center experience of kidney transplantation under tacrolimus immunosuppression.

Transplantation proceedings·2000
Same author

ABO-incompatible living donor kidney transplantation under tacrolimus immunosuppression.

Transplantation proceedings·2000
Same author

Malabsorption of tacrolimus in kidney transplant recipients: a Japanese single center experience.

Transplantation proceedings·2000
Same author

Augmented hepatocellular carcinoma progression and depressed Kupffer cell activity in rat cirrhotic livers.

International journal of oncology·2000
Same author

Adenovirus-mediated gene transfer into tumors: evaluation of direct readministration of an adenoviral vector into subcutaneous tumors of immunocompetent mice.

International journal of oncology·2000
Same author

Effects of naturally occurring stilbene glucosides from medicinal plants and wine, on tumour growth and lung metastasis in Lewis lung carcinoma-bearing mice.

The Journal of pharmacy and pharmacology·2000

Lipase and esterase enzymes share similar catalytic sites. Introducing a hydrophobic site converts esterase into lipase, altering substrate specificity and enzyme function.

Area of Science:

  • Biochemistry
  • Enzymology

Background:

  • Lipases and esterases are enzymes with distinct substrate specificities.
  • Enzyme function is often modulated by specific binding sites.

Purpose of the Study:

  • To investigate the roles of catalytic and hydrophobic recognition sites in lipase and esterase activity.
  • To explore the interconversion between lipase and esterase activities.

Main Methods:

  • Proteolytic digestion of hepatic triglyceride lipase with trypsin.
  • Enzymatic assays using water-insoluble and water-soluble ester substrates.
  • Treatment of human serum carboxylesterase with cardiolipin.

Main Results:

  • Proteolytic digestion altered hepatic triglyceride lipase activity, reducing water-insoluble ester hydrolysis while maintaining water-soluble activity.

Related Experiment Videos

  • Hepatic triglyceride lipase treated with trypsin exhibited esterase-like properties.
  • Cardiolipin enhanced triolein hydrolysis (lipase activity) in carboxylesterase and decreased tributyrin hydrolysis (esterase activity).

    • Conclusions:

    • Lipase and esterase possess similar catalytic sites.
    • The presence of a hydrophobic recognition site is crucial for converting esterase to lipase activity.