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Published on: July 22, 2012
Rapid detection of Van genes in rectal swabs by real time PCR in Southern Brazil
Vlademir Cantarelli1, Bianca Cavalcante, Diogo André Pilger
1Weinmann Laboratório, Porto Alegre, Brazil.
Introduction:
Laboratory-based surveillance is an important component in the control of vancomycin resistant enterococci (VRE).
Methods:
The study aimed to evaluate real-time polymerase chain reaction (RT-PCR) (genes vanA-vanB) for VRE detection on 115 swabs from patients included in a surveillance program.
Results:
Sensitivity of RT-PCR was similar to primary culture (75% and 79.5%, respectively) when compared to broth enriched culture, whereas specificity was 83.1%.
Conclusions:
RT-PCR provides same day results, however it showed low sensitivity for VRE detection.
