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Colony stimulating factor occurs in both inflammatory and noninflammatory synovial fluids
J B Smith1, M H Bocchieri, J B Smith
1Department of Medicine, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Rheumatology International
|January 1, 1990
Summary
Colony stimulating factors (CSF) were detected in synovial fluids from patients with osteoarthritis and rheumatoid arthritis. Macrophage-type CSF was identified, suggesting a role in joint destruction in rheumatological conditions.
Area of Science:
- Rheumatology
- Immunology
- Biochemistry
Background:
- Synovial fluid analysis is crucial for diagnosing and understanding arthritic conditions.
- Colony stimulating factors (CSFs) are key regulators of immune cell production and function.
- The presence and type of CSFs in various arthropathies remain incompletely understood.
Purpose of the Study:
- To investigate the presence and type of colony stimulating factors (CSFs) in synovial fluids from patients with osteoarthritis (OA), rheumatoid arthritis (RA), and other arthritides.
- To correlate CSF levels with disease characteristics, such as inflammation and leukocyte counts.
- To explore the potential role of CSFs in the pathogenesis of joint destruction in rheumatological diseases.
Main Methods:
- Analysis of synovial fluids (SF) from patients with OA, RA, and other arthropathies.
- Detection of colony stimulating factors (CSFs) using biological assays.
- Inhibition studies with monospecific antisera to identify CSF subtypes (e.g., M-CSF).
- Correlation analysis between CSF presence, colony counts, and SF leukocyte levels.
Main Results:
- CSF was detected in 54% of OA SF and 67% of RA SF samples.
- CSF was also found in all septic arthritis samples (5/5).
- Inhibition studies confirmed the presence of macrophage-type CSF (M-CSF) in both RA and OA.
- Significantly higher colony stimulation was observed with RA SF compared to OA SF.
- Higher CSF levels generally correlated with increased SF leukocyte counts.
Conclusions:
- Macrophage-type CSF is present in the synovial fluid of patients with inflammatory and non-inflammatory arthropathies, including OA and RA.
- The levels of CSF and their stimulatory capacity may differ between RA and OA, potentially relating to disease activity.
- These findings suggest that CSFs, alongside other cytokines, may contribute to the joint destruction observed in various rheumatological conditions.