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Related Concept Videos

The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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Mitotic exit control: a space and time odyssey.

Marisa Segal1

  • 1Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK. ms433@cam.ac.uk

Current Biology : CB
|October 29, 2011
PubMed
Summary
This summary is machine-generated.

The mitotic exit network (MEN) controls budding yeast cell cycle. Signals from Tem1 and Cdc5 converge on Cdc15 to ensure MEN activation occurs only at the correct stage of late mitosis.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The mitotic exit network (MEN) is a crucial signaling pathway in budding yeast.
  • MEN controls the timely exit from mitosis, a critical phase of the cell cycle.
  • The small GTPase Tem1 is a key regulator of the MEN pathway.

Purpose of the Study:

  • To elucidate the precise mechanisms by which MEN activation is restricted to late mitosis.
  • To investigate the convergence of regulatory signals onto key MEN components.
  • To understand the roles of Tem1 and Cdc5 in MEN regulation.

Main Methods:

  • Proteomics analysis
  • Yeast genetics
  • Biochemical assays
  • Cell cycle analysis

Main Results:

  • Signals from both Tem1 and the yeast Polo kinase Cdc5 converge on the MEN kinase Cdc15.
  • This convergence ensures that MEN activation is accurately restricted to late mitosis.
  • Identified specific interactions and regulatory events governing MEN activation.

Conclusions:

  • MEN activation is tightly regulated by the integration of signals from Tem1 and Cdc5.
  • The convergence of these signals onto Cdc15 provides a critical checkpoint for mitotic exit.
  • This regulatory mechanism ensures proper cell cycle progression in budding yeast.