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Updated: May 28, 2026
Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
High resolution melting PCR to differentiate Mycobacterium avium subsp. paratuberculosis"cattle type" and "sheep
Pierre E Douarre1, William Cashman, Jim Buckley
1Cork Institute of Technology, Cork, Ireland.
Abstract:
In this study, we describe a novel HRM-PCR assay that clearly differentiates the two main types of Mycobacterium avium subsp. paratuberculosis (cattle and sheep) based on the polymorphic variation of a previously described tandem repeat. This modern genotyping technique has several advantages over alternative methods, including cost, ease of use and rapidity.
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