Related Experiment Video
Updated: May 28, 2026
Quantifying the Level of 8-oxo-dG Using ELISA Assay to Evaluate Oxidative DNA Damage in MCF-7 Cells
Published on: May 24, 2024
Immunochemical detection of oxidatively damaged DNA
1Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague, Czech Republic. prossner@biomed.cas.cz
Abstract:
Oxidatively damaged DNA is implicated in various diseases, including neurodegenerative disorders, cancer, diabetes, cardiovascular and inflammatory diseases as well as aging. Several methods have been developed to detect oxidatively damaged DNA. They include chromatographic techniques, the Comet assay, (32)P-postlabelling and immunochemical methods that use antibodies to detect oxidized lesions. In this review, we discuss the detection of 8-oxo-7,8-dihydro-29-deoxyguanosine (8-oxodG), the most abundant oxidized nucleoside. This lesion is frequently used as a marker of exposure to oxidants, including environmental pollutants, as well as a potential marker of disease progression. We concentrate on studies published between the years 2000 and 2011 that used enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry to detect 8-oxodG in humans, laboratory animals and in cell lines. Oxidative damage observed in these organisms resulted from disease, exposure to environmental pollutants or from in vitro treatment with various chemical and physical factors.
Insights
Oxidatively damaged DNA, particularly 8-oxo-7,8-dihydro-29-deoxyguanosine (8-oxodG), is linked to numerous diseases. This review focuses on ELISA and immunohistochemistry methods for detecting 8-oxodG from 2000-2011.
Area of Science:
- Biochemistry
- Molecular Biology
- Toxicology
Background:
- Oxidative DNA damage is a significant factor in aging and diseases like cancer and neurodegeneration.
- 8-oxo-7,8-dihydro-29-deoxyguanosine (8-oxodG) is the most prevalent oxidized nucleoside and a key biomarker.
- Detecting 8-oxodG aids in understanding disease mechanisms and exposure to harmful agents.
Purpose of the Study:
- To review methods for detecting 8-oxodG, focusing on ELISA and immunohistochemistry.
- To analyze studies on 8-oxodG detection in humans, animals, and cell lines from 2000-2011.
- To highlight the role of 8-oxodG as a marker for disease and environmental exposure.
Main Methods:
- Focus on enzyme-linked immunosorbent assay (ELISA) for 8-oxodG detection.
- Emphasis on immunohistochemistry techniques for visualizing 8-oxodG.
- Review of studies utilizing these methods in various biological models.
Main Results:
- ELISA and immunohistochemistry are effective for detecting 8-oxodG.
- Studies show increased 8-oxodG levels in disease states and after exposure to pollutants.
- Data from humans, laboratory animals, and cell lines were analyzed.
Conclusions:
- 8-oxodG detection is crucial for assessing oxidative stress and its health implications.
- ELISA and immunohistochemistry are valuable tools for 8-oxodG quantification and localization.
- Further research using these methods can advance understanding of oxidative damage in disease.
