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Purification of a new fungal mannose-specific lectin from Penicillium chrysogenum and its aphicidal properties
Frédéric Francis1, Karimi Jaber, Frédéric Colinet
1Functional & Evolutionary Entomology, Gembloux Ago-Bio Tech, University of Liege, Passage des Déportés 2, B-5030 Gembloux, Belgium. Frederic.Francis@ulg.ac.be
Abstract:
Several Ascomycetes fungi are commonly used in bio-industries and provide available industrial residues for lectin extraction to be valuable. A lectin from Penicillium chrysogenum, named PeCL, was purified from a fungal culture using gel-filtration chromatography column. PeCL was found to be a mannose-specific lectin by haemagglutination activity towards rabbit erythrocyte cells and was visualised on SDS-PAGE gel. Purified PeCL fraction was delivered via artificial diet to Myzus persicae aphid and was demonstrated to be aphicidal at 0.1 % with higher toxic efficiency than a known mannose-binding lectin Concanavalin A (ConA). A fast and efficient way to purify PeCL and a potential use in pest control is described.
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