Related Experiment Videos
Integration host factor is a negative effector of in vivo and in vitro expression of ompC in Escherichia coli
L Huang1, P Tsui, M Freundlich
1Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215.
Abstract:
Integration host factor (IHF) of Escherichia coli is a DNA-binding protein involved in gene expression and other cellular functions in E. coli and some of its bacteriophages and plasmids. We report here that IHF is a direct negative effector of the ompC operon of E. coli. IHF binds to ompC DNA and protects a region of 35 base pairs located upstream from the ompC promoters. The addition of IHF to a purified in vitro transcription system inhibited transcription from two of the three ompC promoters. In vivo experiments suggest that the in vitro results are physiologically relevant. IHF mutants show increased expression of OmpC. In addition, the OmpC- phenotype of certain strains is completely suppressed by a mutation in IHF.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Coordination of Gene Expression Processes in Bacteria
Global Regulatory Systems
Stringent Response in E. coli
Regulation of Bacterial Virulence